Recombinant Human SETD8 293 Cell Lysate


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Cat.No.:  EL-0126
Product Name:  Recombinant Human SETD8 293 Cell Lysate
Description:  Antigen standard for SET domain containing (lysine methyltransferase) 8 (SETD8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Species:  Human
Source:  HEK 293 Cells
Components:  This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Storage:  Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  387893
Official Symbol:  SETD8
Official Full Name:  SET domain containing (lysine methyltransferase) 8
Synonyms:  SETD8; SET domain containing (lysine methyltransferase) 8; N-lysine methyltransferase SETD8; KMT5A; PR Set7; SET07; SET8; PR/SET07; H4-K20-HMTase SETD8; lysine N-methyltransferase 5A; SET domain-containing protein 8; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; histone-lysine N-methyltransferase SETD8; H4-K20-specific histone methyltransferase; H4K20-specific histone methyltransferase splice variant Set8b; PR-Set7;
mRNA Refseq:  NM_020382
Protein Refseq:  NP_065115
MIM:  607240
UniProt ID:  Q9NQR1
Chromosome Location:  12q24.31
Function:  histone-lysine N-methyltransferase activity; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transcription corepressor activity; transferase activity;

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