| Cat.No.: | EAb-0104 |
| Product Name: | Histone H4K8ac Polyclonal Antibody |
| Product Overview: | Polyclonal Antibody to detect H4K8ac in human samples. |
| Antibody Type: | Polyclonal |
| Immunogen: | Polyclonal antibody raised in rabbit against the region of histone H4 containing the acetylated lysine 20 (H4K8ac), using a KLH-conjugated synthetic peptide. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | H4K8ac |
| Purification: | Affinity Purified |
| Appearance: | Colorless liquid |
| Formulation: | In PBS with 0.05% sodium azide and 0.05% ProClin 300. |
| Applications: | Western blot, IF, ELISA, Dot Blot, ChIP. |
| Recommended Dilutions/Conditions: |
WB: 1:200, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IF: 1:500, ELISA: 1:100. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | Western blot: HeLa cells, IF: NIH3T3 cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides. |
| Species Reactivity: | Human |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P68431 |
| Alternative Name: | Histone 4 |
| Scientific Background: | Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. |
| Product Types | ||
| ◆ Synthetic Peptides | ||
| SP-0011 | Histone H4 peptide (1-21), Dimethyl R3, Biotin-labeled | Inquiry |
| ◆ Research Kits | ||
| EKIT-0013 | Acetyl Histone H4K12 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0014 | Acetyl Histone H4K12 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0015 | Acetyl Histone H4K16 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0016 | Acetyl Histone H4K16 Quantification Kit (Fluorometric) | Inquiry |
| Related Gene / Proteins | |||
| H4H18 | H4K12 | H4K16 | H4K20 |
| H4K31 | H4K44 | H4K5 | H4K59 |
| H4K79 | H4K8 | H4K91 | H4R3 |
| H4S1 | H4S47 | H4T30 | H4T80 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
