| Cat.No.: | EAb-0106 |
| Product Name: | H4K5,8,12ac Polyclonal Antibody |
| Product Overview: | Polyclonal Antibody to detect H4K5,8,12ac in human , mouse and wide range of samples. |
| Antibody Type: | Polyclonal |
| Immunogen: | KLH-conjugated synthetic peptide of Histone H4 containing acetylated lysines 5, 8 and 12. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | H4K5,8,12ac |
| Purification: | Affinity purified |
| Appearance: | Colorless liquid |
| Formulation: | In PBS with 0.05% sodium azide and 0.05% ProClin 300. |
| Applications: | Western blot, IF, ELISA, ChIP, Dot blot. |
| Recommended Dilutions/Conditions: |
WB:1:1000, ELISA:1:1000, Dot Blot:1:20,000, IF:1:500, ChIP:0.5-1 µg/ChIP. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | Western blot: Whole cell and histone extracts, IF: U2OS cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides. |
| Species Reactivity: | Human, mouse, wide range |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P68431 |
| Alternative Name: | Histone H4 |
| Scientific Background: | Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active genes. |
| Product Types | ||
| ◆ Synthetic Peptides | ||
| SP-0011 | Histone H4 peptide (1-21), Dimethyl R3, Biotin-labeled | Inquiry |
| ◆ Research Kits | ||
| EKIT-0013 | Acetyl Histone H4K12 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0014 | Acetyl Histone H4K12 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0015 | Acetyl Histone H4K16 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0016 | Acetyl Histone H4K16 Quantification Kit (Fluorometric) | Inquiry |
| Related Gene / Proteins | |||
| H4H18 | H4K12 | H4K16 | H4K20 |
| H4K31 | H4K44 | H4K5 | H4K59 |
| H4K79 | H4K8 | H4K91 | H4R3 |
| H4S1 | H4S47 | H4T30 | H4T80 |
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