Cat.No.: | EAb-0134 |
Product Name: | H3 Pan Polyclonal Antibody |
Product Overview: | Polyclonal Antibody to detect H3 Pan in human samples. |
Antibody Type: | Polyclonal |
Immunogen: | KLH-conjugated synthetic peptide of Histone H3 containing an unmodified sequence from the central part and from the C-terminus of histone H3. |
Host: | Rabbit |
Isotype: | IgG |
Antibody Target: | H3 Pan |
Purification: | Whole antiserum from rabbit |
Appearance: | Colorless liquid |
Formulation: | In PBS with 0.05% (W/V) sodium azide. |
Applications: | Western blot, ELISA, ChIP, Dot blot. |
Recommended Dilutions/Conditions: |
WB: 1:250, ELISA: 1:100-1:1000, Dot Blot: 1:1000, ChIP: 1 - 5 µl/ChIP. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
Positive Control: | WB: HeLa cells, ELISA: Antigen, ChIP: Human osteosarcoma cells, Dot blot: Histone peptides |
Species Reactivity: | Human |
Shipping: | Gel Pack |
Storage: | -20°C |
Warning: | For Research Use Only! Not For Use in Humans. |
Accession: | P68431 |
Alternative Name: | Histone H3 |
Scientific Background: | Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. |
Product Types | ||
◆ Nucleosomes | ||
NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
◆ Synthetic Peptides | ||
SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
SP-0010 | Histone H4 peptide (1-21) | Inquiry |
SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
Related Gene / Proteins | |||
HIC1 | HIF1A | HIF1AN | HINFP |
HIPK1 | HIPK2 | HIRA | Histone |
Histone H1 | Histone H2A | Histone H2B | Histone H3 |
Histone H4 | HIV-1 reverse transcriptase |
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