| Cat.No.: | EAb-0260 |
| Product Name: | N6-Methyladenosine Monoclonal Antibody (17-3-4-1) |
| Description: | N6-Methyladenosine Monoclonal Antibody (Clone 17-3-4-1) can be used for dot blot, ELISA, and immunoprecipitation applications. |
| Antibody Type: | Monoclonal |
| Clone Designation: | 17-3-4-1 |
| Immunogen: | N6-Methyladenosine conjugated to BSA |
| Host: | Mouse |
| Isotype: | IgG1k |
| Specificity: | Recognizes RNA or single stranded DNA that is methylated on the adenosine base at the nitrogen-6 position. |
| Purification: | Purified from hybridoma tissue culture supernatant. Protein G affinity purified. |
| Appearance: | Liquid |
| Formulation: | 100 µg of protein A-purified antibody |
| Applications: | Dot blot, ELISA, IP |
| Shipping: | Wet ice |
| Storage: | 4°C |
| Storage Buffer: | 100 µl of PBS, pH 7.2, with 0.02% sodium azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | m6A |
| Scientific Background: | N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 and METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. The process shares similarities to histone methylation in that the modification is installed by m6A methylation “writers”, detected by methylation-specific “readers”, and can be reversed by demethylation “erasers”. The m6A modification has been shown to be conserved in the vicinity of stop codons and the 3’-untranslated region of specific mouse and human mRNAs. The process of regulating m6A modifications in mammalian mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been reported to be an obesity risk gene. FTO is an m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0260 | N6-Methyladenosine Monoclonal Antibody (17-3-4-1) | Inquiry |
| EAb-1987 | N6-Methyladenosine (m6A) Monoclonal Antibody | Inquiry |
| EAb-1988 | N6-Methyladenosine (m6A) Polyclonal Antibody | Inquiry |
| ◆ Research Kits | ||
| EKIT-0311 | m6A RNA Methylation Assay Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| m6A | |||
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