| Cat.No.: | EAb-0487 |
| Product Name: | Histone H3 [Asym-dimethyl Arg8] Polyclonal Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | Synthetic asymmetric dimethylated peptide surrounding Arginine 8 of human Histone H3.2 [Swiss Prot# Q71DI3]. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | HIST2H3C, Asym-dimethyl Arg8 |
| Purification: | Immunogen affinity purified |
| Appearance: | Liquid |
| Formulation: | PBS and 30% Glycerol |
| Applications: | WB, ChIP, ICC/IF, IHC-P |
| Recommended Dilutions/Conditions: |
Western Blot 1:500; Chromatin Immunoprecipitation 2-5 ug per million cells; Immunocytochemistry/Immunofluorescence 1:200; Immunohistochemistry-Paraffin Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Mouse, C. Elegans, Rat, Chicken, Drosophilia, Plant, Xenopus |
| Storage: | -20°C |
| Storage Buffer: | 0.05% Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | H3 histone, family 3A; H3.3AH3F3H3F3B; H3.3B; H3F3A; Histone H3; histone H3.3; MGC87782; MGC87783 |
| Scientific Background: | Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimethylation of H3 Arg8 (H3 R8Me2) is known as a mark of transcriptional repression. The protein arginine methyltransferases PRMT5 and PRMT2 can both methylate Arg8, with PRMT2 specifically methylating in an asymmetric manner (PMID 20708585). In addition, asymmetric dimethylation of Arg8 inhibits H3K9 methylation by G9a, but not symmetric Arg8Me2 does not (PMID 18438403). |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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