| Cat.No.: | EAb-0506 |
| Product Name: | Histone H3 Monoclonal Antibody (1B1-B2) |
| Antibody Type: | Monoclonal |
| Clone Designation: | 1B1-B2 |
| Immunogen: | Synthetic peptide corresponding to the C-terminus of human Histone H3. |
| Host: | Mouse |
| Isotype: | IgG3 Kappa |
| Antibody Target: | HIST2H3C |
| Purification: | Protein G purified |
| Appearance: | Liquid |
| Formulation: | PBS |
| Applications: | WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-P, Single Cell Western |
| Recommended Dilutions/Conditions: |
Western Blot 1:1000; Chromatin Immunoprecipitation; Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry 1:200-1:500; Immunohistochemistry-Paraffin 1:200-1:500; Flow (Intracellular); Single Cell Western 100 ug/ml Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Mouse, Rat |
| Storage: | -20°C |
| Storage Buffer: | 0.02% Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | H3 histone, family 3A; H3.3AH3F3H3F3B; H3.3B; H3F3A; Histone H3; histone H3.3; MGC87782; MGC87783 |
| Scientific Background: | Eukaryotic chromatin exists as nucleosomes, each of which is composed of an octamer of histone molecules, around which the DNA is wrapped. Each octamer contains two molecules each of histones H2A, H2B, H3 and H4. Histone proteins consist of a globular domain and a flexible N-terminal tail; the tail protrudes from the nucleosome and is the site of a huge diversity of post-translational modifications that support the maintenance of an active or inactive transcriptional state. Numerous modifications of histone H3 are known, including methylation or acetylation of arginine or lysine residues, and phosphorylation of serine or threonine residues; these modifications may promote or inhibit transcription. There are three variants of histone H3, known as H3.1, H3.2 and H3.3, each of which is encoded by a number of different genes. Although these variants share similar amino acid sequences, they differ in their expression pattern and post-translational modifications and perform distinct functions. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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