| Cat.No.: | EAb-0571 |
| Product Name: | Histone H3 K18ac Polyclonal Antibody |
| Product Overview: | Rabbit polyclonal to detect Histone H3 (acetyl K18) |
| Antibody Type: | Polyclonal |
| Immunogen: | Prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 18 of human Histone H3.2. |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | Affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Appearance: | Liquid |
| Formulation: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Applications: | Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence |
| Recommended Dilutions/Conditions: |
ChIP: 2-5µg/million cells; IF Microscopy: 1:500; Western Blot: 1:500; Immunohistochemistry: 1:500; Dot Blot: 1:1000 Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Cross Reactivity: | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Shipping: | Dry Ice |
| Storage: | -20°C |
| Storage Buffer: | 0.01% (w/v) Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | UniprotKB - Q71DI3; NCBI - NP_001005464; GeneID - 126961 |
| Alternative Name: | rabbit anti-Histone H3 Ac Lys18 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K18ac |
| Scientific Background: | Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, the acetylation of lysine 18 on histone 3 (H3 K18ac) is associated with transcriptional activation, DNA replication, and DNA repair. Enzymes known to acetylate K18 included Gcn5, p300, CBP, and TFIIIC90. Acetylation of this amino acid is also known to potentiate other arginine methylation sites on H3. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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