| Cat.No.: | EAb-0604 |
| Product Name: | Histone H3pT11 Polyclonal Antibody |
| Product Overview: | Anti-Histone H3 [p Thr11] (RABBIT) Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | Prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Threonine 11 of human Histone H3.2. |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | Affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Appearance: | Liquid |
| Formulation: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Applications: | Western Blot, Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, and Immunofluorescence |
| Recommended Dilutions/Conditions: |
ChIP: 2-5µg/million cells; IF Microscopy: 1:50; Western Blot: 1:1000; Immunohistochemistry: 1:50; Dot Blot: 1:1000 Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Cross Reactivity: | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Shipping: | Dry Ice |
| Storage: | -20°C |
| Storage Buffer: | 0.01% (w/v) Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | UniprotKB - Q71DI3; NCBI - NP_001005464; GeneID - 126961 |
| Alternative Name: | rabbit anti-Histone H3 pT11 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3pT11 |
| Scientific Background: | Phosphorylation of H3 T11 is most abundant in the centromere, and initiates centromere assembly in mammalian cells. Dlk/ZIP kinase phosphorylates H3 T11, but may be affected by epigenetic modification itself during cellular interphase, which changes the rate of phosphorylation and de-phosphorylation of H3 T11. Other epigenetic modifiers also interact with H3 T11, including the histone acetyltransferase GCN5, which binds more readily to pT11 than unphosphorylated peptide. This binding affects downstream regulation of GCN5 genes and can contribute to transcriptional regulation. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
| Related Gene / Proteins | |||
| H3K10 | H3K115 | H3K122 | H3K123 |
| H3K14 | H3K18 | H3K23 | H3K27 |
| H3K36 | H3K37 | H3K4 | H3K5 |
| H3K56 | H3K64 | H3K79 | H3K80 |
| H3K9 | H3R128 | H3R17 | H3R2 More > |
| H3R26 | H3R8 | H3R9 | H3S10 |
| H3S28 | H3S31 | H3S32 | H3T11 |
| H3T118 | H3T3 | H3T32 | H3T41 |
| H3T45 | H3T6 | H3T80 | H3Y41 |
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