| Cat.No.: | EAb-0617 |
| Product Name: | Histone H4 K20me2 Polyclonal Antibody |
| Product Overview: | Anti-Histone H4 [Dimethyl Lys20] (RABBIT) Antibody |
| Antibody Type: | Polyclonal |
| Immunogen: | Prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4. |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | Affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Appearance: | Liquid |
| Formulation: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Applications: | Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry |
| Recommended Dilutions/Conditions: |
ChIP: 2-5µg/million cells; IF Microscopy: 1:50; Western Blot: 1:2000; Immunohistochemistry: 1:50; Dot Blot: 1:1000 Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Cross Reactivity: | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Shipping: | Dry Ice |
| Storage: | -20°C |
| Storage Buffer: | 0.01% (w/v) Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | UniprotKB - P62805; NCBI - NP_001029249; GeneID - 121504 |
| Alternative Name: | rabbit anti-Histone H4 dimethyl Lys20 antibody, H4K20me2, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4 |
| Scientific Background: | When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information. |
| Product Types | ||
| ◆ Synthetic Peptides | ||
| SP-0011 | Histone H4 peptide (1-21), Dimethyl R3, Biotin-labeled | Inquiry |
| ◆ Research Kits | ||
| EKIT-0013 | Acetyl Histone H4K12 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0014 | Acetyl Histone H4K12 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0015 | Acetyl Histone H4K16 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0016 | Acetyl Histone H4K16 Quantification Kit (Fluorometric) | Inquiry |
| Related Gene / Proteins | |||
| H4H18 | H4K12 | H4K16 | H4K20 |
| H4K31 | H4K44 | H4K5 | H4K59 |
| H4K79 | H4K8 | H4K91 | H4R3 |
| H4S1 | H4S47 | H4T30 | H4T80 |
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