| Cat.No.: | EAb-2178 |
| Product Name: | Histone H4K12ac Monoclonal Antibody |
| Antibody Type: | Monoclonal |
| Immunogen: | This antibody was raised against a synthetic peptide containing acetyl-lysine 12 of human Histone H4. |
| Host: | Mouse |
| Isotype: | IgG1 |
| Molecular Weight: | 8 kDa |
| Purification: | Protein G Chromatography |
| Appearance: | Liquid |
| Formulation: | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| Applications: | ChIP: 2 - 5 µg per ChIP; WB: 0.5 - 2 µg/ml dilution^Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Wide Range Predicted |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Scientific Background: | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation. |
| Product Types | ||
| ◆ Synthetic Peptides | ||
| SP-0011 | Histone H4 peptide (1-21), Dimethyl R3, Biotin-labeled | Inquiry |
| ◆ Research Kits | ||
| EKIT-0013 | Acetyl Histone H4K12 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0014 | Acetyl Histone H4K12 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0015 | Acetyl Histone H4K16 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0016 | Acetyl Histone H4K16 Quantification Kit (Fluorometric) | Inquiry |
| Related Gene / Proteins | |||
| H4H18 | H4K12 | H4K16 | H4K20 |
| H4K31 | H4K44 | H4K5 | H4K59 |
| H4K79 | H4K8 | H4K91 | H4R3 |
| H4S1 | H4S47 | H4T30 | H4T80 |
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