Cat.No.: | EAb-2335 |
Product Name: | Histone H3K27me1 Polyclonal Antibody |
Product Overview: | Rabbit polyclonal to detect Histone H3 (mono methyl K27) |
Antibody Type: | Polyclonal |
Immunogen: | This Histone H3 monomethyl Lys27 antibody was raised against a peptide including monomethyl-lysine 27 of human histone H3. |
Host: | Rabbit |
Isotype: | Serum |
Molecular Weight: | 17 kDa |
Purification: | None |
Appearance: | Liquid |
Formulation: | Rabbit serum containing 30% glycerol and 0.035% sodium azide. |
Applications: | ICC/IF: 1:500 - 1:2,000 dilution; WB: 1:500 - 1:2,000 dilution^Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
Species Reactivity: | Human, Wide Range Predicted |
Storage: | -20°C |
Warning: | For Research Use Only! Not For Use in Humans. |
Scientific Background: | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression. |
Product Types | ||
◆ Research Kits | ||
EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
Related Gene / Proteins | |||
H3K10 | H3K115 | H3K122 | H3K123 |
H3K14 | H3K18 | H3K23 | H3K27 |
H3K36 | H3K37 | H3K4 | H3K5 |
H3K56 | H3K64 | H3K79 | H3K80 |
H3K9 | H3R128 | H3R17 | H3R2 More > |
H3R26 | H3R8 | H3R9 | H3S10 |
H3S28 | H3S31 | H3S32 | H3T11 |
H3T118 | H3T3 | H3T32 | H3T41 |
H3T45 | H3T6 | H3T80 | H3Y41 |
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