| Cat.No.: | PE-0123 |
| Product Name: | Recombinant Human SIRT1, GST-tagged |
| Product Overview: | Recombinant Human SIRT1 (amino acids 193- 741) was expressed in an E.coli expression system with N-terminal GST tag. MW=87.2 kDa. |
| Description: | Sirtuin 1 also known as NAD-dependent deacetylase sirtuin-1 is a protein that in humans is encoded by the SIRT1 gene. SIRT1 stands for sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), referring to the fact that its sirtuin homolog (biological equivalent across species) in yeast (S. cerevisiae) is Sir2. SIRT1 is an enzyme which deacetylates proteins that contribute to cellular regulation (reaction to stressors, longevity). |
| Applications: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | > 80%. |
| Species: | Human |
| Formulation: | 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol. |
| Tag: | GST |
| Activity: | 8 pmol/min /μg. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 μM Biomol substrate, 200 μM NAD+and 20 ng/μl Sirtuin 1. Incubation condition: 30 min at 30℃. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SIRT1 sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) [ Homo sapiens ] |
| Gene ID NCBI: | 23411 |
| Synonyms: | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); SIR2L1; SIRT1; sirtuin 1; SIR2alpha; sir2-like 1; sirtuin type 1; EC 3.5.1.-; OTTHUMP00000019691; OTTHUMP00000060745; SIR2alpha; hSIR2; hSIRT1; SIR2-like protein 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) |
| mRNA Refseq: | NM_001142498 |
| Protein Refseq: | NP_001135970 |
| MIM: | 604479 |
| Chromosome Location: | 10q21.3 |
| Function: | NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; deacetylase activity; histone binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; identical protein binding; metal ion binding; p53 binding; protein C-terminus binding; protein domain specific binding; transcription corepressor activity; zinc ion binding |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
