| Cat.No.: | PE-0126 |
| Product Name: | Recombinant Human Sirtuin 1, His-tagged |
| Product Overview: | Recombinant human SIRT1 (193-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. |
| Description: | SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. It is an NAD (+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. |
| Applications: | SIRT Assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 84 kDa |
| Purity: | > 70 % |
| Species: | Human |
| Formulation: | Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. |
| Tag: | His |
| Activity: | 2100 RLU/min/mg |
| Expression System: | Sf9 Insect Cells |
| Protein Length: | 193-end |
| Concentrations: | 0.1 ug/ul |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SIRT1 sirtuin 1 [ Homo sapiens ] |
| Gene ID NCBI: | 23411 |
| Official Symbol: | SIRT1 |
| Synonyms: | SIRT1; sirtuin 1; SIR2L1; NAD-dependent deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; OTTHUMP00000019691; OTTHUMP00000198112; SIR2-like protein 1; EC 3.5.1.-; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1 |
| mRNA Refseq: | NM_001142498 |
| Protein Refseq: | NP_001135970 |
| MIM: | 604479 |
| UniProt ID: | Q96EB6 |
| Chromosome Location: | 10q21 |
| Function: | NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; deacetylase activity; histone binding; hydrolase activity; identical protein binding; metal ion binding; p53 binding; protein C-terminus binding; protein domain specific binding; transcription corepressor activity; zinc ion binding; HLH domain binding |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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