| Cat.No.: | PE-0155 |
| Product Name: | Recombinant Human Sirtuin 3, His-tagged |
| Product Overview: | Recombinant human SIRT3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =33.5kDa (303aa) confirmed by MALDI-TOF. |
| Description: | NAD-dependent deacetylase sirtuin-3, mitochondrial, also known as SIRT3, belongs to the sirtuin family of proteins. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. SIRT3 exhibits NAD+-dependent deacetylase activity in the mitochondria. Over-expression of SIRT3 results in increased levels of the mitochondrial uncoupling protein 1. SIRT3 protein levels are also elevated in certain breast cancers. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | > 95% by SDS – PAGE. |
| Species: | Human |
| Formulation: | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl. |
| Tag: | His |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSDKGKLSLQ DVAELIRARA CQRVVVMVGA GISTPSGIPD FRSPGSGLYS NLQQYDLPYP EAIFELPFFF HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FASLTEAVRS SVPRLLINRD LVGPLAWHPR SRDVAQLGDV VHGVESLVEL LGWTEEMRDL VQRETGKLDG PDK |
| Expression System: | E. coli |
| Concentrations: | 1 mg/ml (determined by Bradford assay). |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SIRT3 sirtuin 3 [ Homo sapiens ] |
| Gene ID NCBI: | 23410 |
| Synonyms: | SIRT3; sirtuin 3; SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin type 3 |
| mRNA Refseq: | NM_001017524 |
| Protein Refseq: | NP_001017524 |
| MIM: | 604481 |
| UniProt ID: | Q9NTG7 |
| Chromosome Location: | 11p15.5 |
| Function: | NAD binding; NAD+ ADP-ribosyltransferase activity; hydrolase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; metal ion binding; protein binding; zinc ion binding |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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