| Cat.No.: | PE-0580 |
| Product Name: | Recombinant Human ATXN3L, His-tagged |
| Product Overview: | Recombinant human ATXN3L (amino acid residues 1-355), fused with N-terminal His, was expressed in E.coli. |
| Description: | There are two main classes of DUB; cysteine proteases and metalloproteases. Ataxin-3L is a cysteine protease and a member of the Machado-Joseph Domain (MJD) enzyme family. Cloning of the human gene was first described by Gerhard et al. (2004). Machado–Joseph disease (MJD), the most common form of spinocerebellar ataxia worldwide, is a progressive and ultimately fatal neurodegenerative disorder caused by polyQ expansion in ataxin-3, a conserved and ubiquitous protein known to bind polyubiquitin chains and to function as a deubiquitylating enzyme. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | ~43 kDa |
| Purity: | >92% by SDS-PAGE |
| Species: | Human |
| Formulation: | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
| Tag: | His |
| Activity: | Deubiquitylase Enzyme Assay: The activity of His-Ataxin-3L was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-Ataxin-3L was compared confirming the deubiquitylating activity of His-Ataxin-3L. |
| Expression System: | E. coli |
| Concentrations: | 0.5 mg/ml |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | ATXN3L ataxin 3-like [ Homo sapiens ] |
| Gene ID NCBI: | 92552 |
| Synonyms: | ataxin 3-like; ATX3L; MJDL; EC 3.4.19.12; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein |
| mRNA Refseq: | NM_001135995.1 |
| Protein Refseq: | NP_001129467.1 |
| UniProt ID: | B4DYC7 |
| Chromosome Location: | Xp22.2 |
| Function: | omega peptidase activity; ubiquitin-specific protease activity |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0020 | Human ATAD2 Knockout Cell Line 2bp deletion | Inquiry |
| CL-0021 | Human ATAD2B Knockout Cell Line 130bp insertion | Inquiry |
| CL-0022 | Human ATAD2B Knockout Cell Line 130bp insertion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0021 | ATF2 (pT69/pT71) Transcription Factor Assay Kit | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0031 | Recombinant Human ATAD2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| ATAD2 | ATAT1 | ATF-6 | ATF1 |
| ATF2 | ATF4 | ATM | ATR |
| ATRX | ATXN3 | ATXN3L | ATXN7 |
| ATXN7L3 | |||
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
