| Cat.No.: | PE-0582 |
| Product Name: | Recombinant Human ATXN3L protein, His-tagged |
| Product Overview: | Recombinant Human ATXN3L fused with His tag at C-terminal was expressed in Insect cells. |
| Description: | Ataxin-3-Like protein, also known as Machado-Joseph Disease (MJD) Protein-1-Like, is a 355 amino acid deubiquitinating (DUB) enzyme with a predicted molecular weight of 41 kDa. Full-length Ataxin-3-L contains an N-terminal Joesephin Domain, two Ubiquitin-Interacting-Motifs (UIM's) and a C-terminus consisting of a polyglutamine stretch and tail. Ataxin-3-L targets include Kruppel-Like Factor 5 (KLF5). KLF5 has been reported to promote breast cell proliferation, survival and tumorigenesis. Ubiquitination and proteasome-mediated degradation of KLF5 is antagonized by Ataxin-3-L, suggesting that inhibition of this DUB might provide a target for therapeutic intervention in breast cancer. Ataxin-3 and Ataxin-3-L have been reported to cleave both K48- and K63-linked poly-Ubiquitin in vitro, though enzyme activity in such assays is inefficient when using shorter chain lengths (≤ 6). Ataxin-3-L activity can be efficiently monitored with K63-linked tetra-Ubiquitin Rhodamine (UC-355) substrate. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | Predicted Molecular Mass: 42 kDa |
| Purity: | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. |
| Species: | Human |
| Formulation: | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Tag: | His |
| Activity: | Recombinant Human His6-Ataxin-3-Like protein is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-Ataxin-3-like concentration of 10-100 nM when utilizing K63-linked tetra-Ubiquitin Rhodamine. Higher concentrations may be required for other substrates. |
| Expression System: | Insect Cells |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | ATXN3L ataxin 3-like [ Homo sapiens ] |
| Gene ID NCBI: | 92552 |
| Official Symbol: | ATXN3L |
| Synonyms: | ataxin 3-like; ATX3L; MJDL; EC 3.4.19.12; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein |
| mRNA Refseq: | NM_001135995.1 |
| Protein Refseq: | NP_001129467.1 |
| UniProt ID: | B4DYC7 |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0020 | Human ATAD2 Knockout Cell Line 2bp deletion | Inquiry |
| CL-0021 | Human ATAD2B Knockout Cell Line 130bp insertion | Inquiry |
| CL-0022 | Human ATAD2B Knockout Cell Line 130bp insertion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0021 | ATF2 (pT69/pT71) Transcription Factor Assay Kit | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0031 | Recombinant Human ATAD2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| ATAD2 | ATAT1 | ATF-6 | ATF1 |
| ATF2 | ATF4 | ATM | ATR |
| ATRX | ATXN3 | ATXN3L | ATXN7 |
| ATXN7L3 | |||
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