| Cat.No.: | PE-0682 |
| Product Name: | Recombinant Human Sp1 Transcription Factor, His-tagged |
| Description: | The his-tagged wild type Sp1 protein (785 amino acids, 100 kDa) was expressed in a baculovirus system and purified by affinity column and FPLC.Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNAbinding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a wellcharacterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease, and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation. |
| Applications: | Sp1 can be used for in vitro transcripion activation, DNAse I activation and gel mobility shift assays. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | > 90% by SDS-PAGE. |
| Species: | Human |
| Formulation: | Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol. |
| Tag: | His |
| Activity: | 30-100 ng are required for a reconstituted transcription assay and 5-25 ng are required for a gel mobility shift assay in a 20 μl reaction. |
| Expression System: | Sf9 Insect Cells |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SP1 Sp1 transcription factor [ Homo sapiens ] |
| Gene ID NCBI: | 6667 |
| Synonyms: | SP1; Sp1 transcription factor; Sp1 transcription factor; specificity protein 1; TSFP1;Transcription factor Sp1 |
| mRNA Refseq: | NM_003109 |
| Protein Refseq: | NP_003100 |
| MIM: | 189906 |
| UniProt ID: | P08047 |
| Chromosome Location: | 12q13.1 |
| Function: | RNA polymerase II transcription factor activity; double-stranded DNA binding; histone deacetylase binding; metal ion binding; promoter binding; protein C-terminus binding; protein homodimerization activity; transcription activator activity; transcription factor activity; zinc ion binding |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0089 | Recombinant Human SP2 Cell Lysate | Inquiry |
| ◆ Cell Lines | ||
| CL-0161 | Human SP1 Knockout Cell Line 2bp deletion | Inquiry |
| CL-0169 | Human SP140L Knockout Cell Line | Inquiry |
| CL-0170 | Human SP100 Knockout Cell Line 20bp deletion | Inquiry |
| CL-0171 | Human SP110 Knockout Cell Line 7bp deletion | Inquiry |
| Related Gene / Proteins | |||
| SP1 | SP100 | SP110 | SP140 |
| SP140L | SP2 | SP3 | SP6 |
| SPDL1 | SPF30 | SPIN1 | SPOP |
| SPT16 | SPT3 | Spt6 | SPTY2D1 |
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