| Cat.No.: | PE-1131 |
| Product Name: | Recombinant Human NCOA1 protein, Biotinylated |
| Product Overview: | The His-tag biotinylated recombinant protein is purified by affinity chromatography in combination with FPLC columns. Then His-tag was removed by protease. |
| Description: | Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family. SRC1 has been shown to be over expressed in some cancers. SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction. The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions. This protein does not contain His tag and will be useful for assays where a native untagged protein is desired. Lane 1 corresponds to 6His-tagged and biotinylated SRC-RID; Lane 2 corresponds to biotinylated SRC-RID without 6His tag. |
| Applications: | The biotinylated SCR1-RID (627-786) can be used for protein-protein interaction assays, particularly suitable for high throughput screening (HTS) assays. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 19.4 kDa |
| Purity: | Purified biotinylated SRC1-RID is greater than 90% homogeneous based on SDS-PAGE analysis. |
| Species: | Human |
| Formulation: | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. |
| Amino Acid Sequence: | SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTERHKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDHQLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT |
| Expression System: | E. coli |
| Protein Length: | 627-786 aa |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | NCOA1 nuclear receptor coactivator 1 [ Homo sapiens ] |
| Gene ID NCBI: | 8648 |
| Official Symbol: | NCOA1 |
| Synonyms: | NCOA1; nuclear receptor coactivator 1; bHLHe74; F SRC 1; KAT13A; NCoA 1; RIP160; SRC1; Hin-2 protein; PAX3/NCOA1 fusion protein; steroid receptor coactivator-1; renal carcinoma antigen NY-REN-52; class E basic helix-loop-helix protein 74; F-SRC-1; bHLHe42; MGC129719; MGC129720; |
| mRNA Refseq: | NM_003743 |
| Protein Refseq: | NP_003734 |
| MIM: | 602691 |
| UniProt ID: | Q15788 |
| Chromosome Location: | 2p23 |
| Function: | DNA binding; androgen receptor binding; chromatin binding; enzyme binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein N-terminus binding; protein binding; signal transducer activity |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0046 | HDAC3/NCOR1 fluorometric drug discovery Kit | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0046 | Recombinant Human NCOR1, GST-tagged | Inquiry |
| ◆ Antibodies | ||
| EAb-0065 | NCOA1 Polyclonal Antibody | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0143 | Recombinant Human NCOA1 293 Cell Lysate | Inquiry |
| EL-0144 | Recombinant Human NCOA2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| NCAPD3 | NCAPH2 | NCOA1 | NCOA2 |
| NCOA3 | NCoA4 | NCOR1 | ncor2 |
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