| Cat.No.: | PE-1234 |
| Product Name: | Recombinant Human E1A Binding Protein P300, 302-531aa |
| Description: | Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as tumor suppressor. The ability of p300 to acetylate many transcription factors, including p53, E2F, TFIIE, and TFIIF etc. demonstrated a novel mechanism of targeted p300 regulation of gene expression. |
| Applications: | Use only for research. Recombinant p300-Ch1 can be used for 1) protein-protein interaction assay, 2) in vitro transcription assay, 3) in vitro acetylation assay and 4) cell growth assay. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | > 95% by SDS-PAGE. |
| Species: | Human |
| Formulation: | Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA and 1 mM DTT. |
| Activity: | 1-5 ng are sufficient for a gel mobility shift assay in a 20µl reaction, 50-100 ng are sufficient for reconstituted transcription assay and 100-200 ng are sufficient for a protein-protein interaction assay or an acetylation assay. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | EP300 E1A binding protein p300 [ Homo sapiens ] |
| Gene ID NCBI: | 2033 |
| Synonyms: | EP300; E1A binding protein p300; p300; KAT3B; E1A-binding protein, 300kD; E1A-associated protein p300; histone acetyltransferase p300; EC 2.3.1.48; p300 HAT; OTTHUMP00000028668; P300 |
| mRNA Refseq: | NM_001429 |
| Protein Refseq: | NP_001420 |
| MIM: | 602700 |
| UniProt ID: | Q09472 |
| Chromosome Location: | 22q13.2 |
| Function: | acetyltransferase activity; histone acetyltransferase activity; metal ion binding; peroxisome proliferator activated receptor binding; protein C-terminus binding; transcription coactivator activity; transcription factor activity; transferase activity; zinc ion binding |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0038 | CTPB | Inquiry |
| BSM-0073 | Anacardic Acid | Inquiry |
| BSM-0094 | C646 | Inquiry |
| BSM-0108 | Curcumin, Curcuma longa (High Purity) | Inquiry |
| ◆ Cell Lines | ||
| CL-0061 | Human EP300 Knockout Cell Line 13bp deletion | Inquiry |
| Related Gene / Proteins | |||
| EP300 | EPC1 | ||
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