| Cat.No.: | PE-1478 |
| Product Name: | Recombinant Human Centromere Protein A, His-tagged |
| Product Overview: | Recombinant Human CENP-A produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENP-A is expressed with a -6x His tag and purified by proprietary chromatographic techniques |
| Description: | Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-A is another important centromeric autoantigen in addition to CENP-B: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENP-A antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENP-B-autoantibody negative patients. |
| Applications: | Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Sterile Filtered liquid formulation. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Species: | Human |
| Formulation: | CENP-A is supplied at a concentration of 0.6 mg/ml in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea. |
| Tag: | His |
| Expression System: | Sf9 Insect Cells |
| Concentrations: | 0.15-0.4 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | CENPA centromere protein A [ Homo sapiens ] |
| Gene ID NCBI: | 1058 |
| Synonyms: | centromere protein A; CENP-A; CENPA; centromere protein A, 17kDa; centromere protein A (17kD); OTTHUMP00000122589; Centromere autoantigen A |
| mRNA Refseq: | NM_001042426 |
| Protein Refseq: | NP_001035891 |
| MIM: | 117139 |
| UniProt ID: | P49450 |
| Chromosome Location: | 2p24-p21 |
| Function: | DNA binding; chromatin binding; protein binding |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0054 | Human CECR2 Knockout Cell Line 13bp deletion | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0191 | Human CENPA peptide | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0219 | Recombinant Human CENPA 293 Cell Lysate | Inquiry |
| EL-0220 | Recombinant Human CENPA 293 Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0340 | NVS-CECR2-1 | Inquiry |
| Related Gene / Proteins | |||
| CEACAM1 | CECR1 | CECR2 | CELF-5 |
| CELF-6 | CENH3 | CENPA | CENPB |
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