| Cat.No.: | PE-1579 |
| Product Name: | Histone Octamer H3K27cMe2, His-tag |
| Product Overview: | Human recombinant histone octamer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, dimethyl aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa. |
| Applications: | A substrate for histone methyltransferases and demethylases. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 116 kDa |
| Species: | Human |
| Formulation: | 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol |
| Tag: | His |
| Expression System: | E. coli |
| Format: | Aqueous buffer solution |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene ID NCBI: | NM_033445, NM_003528, NM_003532, and NM_003548 |
| UniProt ID: | P68431 |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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