| Cat.No.: | NUC-0095 |
| Product Name: | Recombinant Mononucleosomes, Hemi-methylated |
| Description: | Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 187 base pairs of DNA containing the 601 positioning sequence DNA. The the 187 bp DNA sequence contains a 147 base-pair 601 nucleosome positioning sequence. The 601 sequence is flanked by a hemi-methylated 20 bp sequence as shown in application notes. The 601 DNA contains a 5' biotin-TEG group. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 224,650 Da. |
| Formulation: | Mononucleosomes, Recombinant, 187x601 DNA (50 µg DNA + protein, 24.3 µg protein weight) in 52 µl 10 mM Tris pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM EDTA, 20% glycerol. |
| Tag: | Biotin |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0001 | Recombinant Nucleosome | Inquiry |
| NUC-0002 | Native Nucleosome (HeLa-derived) | Inquiry |
| NUC-0003 | Native Nucleosome (HEK293-derived) | Inquiry |
| NUC-0004 | Recombinant Nucleosome | Inquiry |
| NUC-0005 | Nucleosomes (Chicken Oligo/Poly H5 Enriched) | Inquiry |
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