| Cat.No.: | PE-3474 |
| Product Name: | Recombinant EIF4A3 protein |
| Product Overview: | Recombinant EIF4A3 protein was expressed in E.coli as the full length protein (accession number NP_055555.1) with an N-terminal 6×His tag. The molecular weight of the protein is 50.4 kDa. |
| Background: | EIF4A3 (Eukaryotic Translation Initiation Factor 4A, Isoform 3), also called as DDX48, NUK34 or RCPS, is a ATP-dependent RNA helicase, which is involved in pre-mRNA splicing as component of the spliceosome. It’s a core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The RNA-dependent ATPase and RNA-helicase activities of EIF4A3 are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. This protein is involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. EIF4A3 binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. And it shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris-HCl pH 7.4, 300 mM NaCl,5% glycerol, 0.1% Triton X-100. |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Proteins & Enzymes | ||
| PE-2388 | Recombinant Human EID1 protein | Inquiry |
| PE-2389 | Recombinant Human EID1 protein | Inquiry |
| PE-3474 | Recombinant EIF4A3 protein | Inquiry |
| PE-3475 | Recombinant EIF4A2 protein | Inquiry |
| PE-3476 | Recombinant EIF4A1 protein | Inquiry |
| Related Gene / Proteins | |||
| EID1 | EIF4A1 | EIF4A2 | EIF4A3 |
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