| Cat.No.: | PE-3489 |
| Product Name: | Recombinant KDM7A protein |
| Product Overview: | Recombinant KDM7A (accession number NP_085150.1) was expressed in Sf9 and contains an N-terminal FLAG-Tag with a molecular weight of 109.6 kDa. The recombinant protein is >70% pure by SDS-PAGE. |
| Background: | KDM7A (Lysine (K)-specific demethylase 7A), also known as Jumonji C Domain Containing Histone Demethylase 1 Homolog D (JHDM1D), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. KDM7A has two N-terminal domains, a PHD finger that binds tri-methylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2) and Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression). The PHD domain of KDM7A specifically binds H3K4me3, affecting histone demethylase specificity. In the presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. It also demethylates H3K9me2 in absence of H3K4me3. KDM7A demethylates H4K20me1 only when nucleosome is used as a substrate. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
| Tag: | FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
| CL-0089 | Human KDM2B Knockout Cell Line 14bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A More > |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | ||
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