| Cat.No.: | NUC-0149 |
| Product Name: | Human Recombinant Nucleosome |
| Description: | Recombinant nucleosome, assembled in vitro by salt dialysis using the 147 bp 601 DNA sequence and the fourcore histones, H2A, H2B, H3 and H4, produced in E. Coli. Suitable for in vitro chromatin assembly and enzyme activity assays. |
| Background: | The nucleosome is the smallest part of the chromosome. It consists of two core histones of each class H2A, H2B, H3 and H4which assemble and are wrapped around 146 base pairs of DNA. Histones are the main constituents of the protein part ofchromosomes of eukaryotic cells. They pack the DNA into tight masses of chromatin. Histones are rich in the amino acidsarginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translationalmodifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression orother nuclear processes. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Purity: | Purified using FPLC, >98% purity as determined by SDS-PAGE |
| Species: | Human |
| Formulation: | 20 mM Tris-Cl pH 7.9, 1mM EDTA, 0.5 mMPMSF, 1 mM DTT and 40% glycerol. |
| Expression System: | E. coli |
| Concentrations: | 0.25 µg/µl |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0001 | Recombinant Nucleosome | Inquiry |
| NUC-0002 | Native Nucleosome (HeLa-derived) | Inquiry |
| NUC-0003 | Native Nucleosome (HEK293-derived) | Inquiry |
| NUC-0004 | Recombinant Nucleosome | Inquiry |
| NUC-0005 | Nucleosomes (Chicken Oligo/Poly H5 Enriched) | Inquiry |
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