Cat.No.: | EKIT-0202 |
Product Name: | HDAC1 Immunoprecipitation (IP) & Activity Assay Kit |
Size: | 25 assays |
Product Overview: | Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader. |
Kit Components: | HDAC Assay Buffer; Cell Lysis Buffer; HDAC Substrate; Developer; AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM); Rabbit HDAC2 Antibody (0.2 mg/ml); Rabbit IgG (Control Antibody) (0.5 mg/ml); Protein A/G Sepharose Beads; Positive Control (Jurkat Cell Lysate) (125 μg) |
Features & Benefits: | Simple, fast and convenient. Only kit on the market for both IP as well as measuring activity of HDAC1 |
Detection Method: | Fluorescence (Ex/Em=380/500 nm) |
Shipping: | Gel Pack |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Extracts & Lysates | ||
EL-0006 | Recombinant Human HDAC1 293 Cell Lysate | Inquiry |
EL-0007 | Recombinant Human HDAC2 293 Cell Lysate | Inquiry |
EL-0008 | Recombinant Human HDAC3 Cell Lysate | Inquiry |
EL-0009 | Recombinant Human HDAC4 Cell Lysate | Inquiry |
◆ Bioactive Small Molecules | ||
BSM-0007 | Tubacin | Inquiry |
Related Gene / Proteins | |||
HDAC | HDAC1 | HDAC10 | HDAC11 |
HDAC2 | HDAC3 | hdac4 | hdac5 |
HDAC6 | hdac7 | HDAC8 | hdac9 |
USA
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