| Cat.No.: | EKIT-0426 |
| Product Name: | SREBP-1 Transcription Factor Assay Kit |
| Assay Type: | Semi-quantitative |
| Description: | SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate. SREBP-1 contained in a nuclear extract binds specifically to the SREBP response element. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The SREBP-1 Transcription Factor Assay detects human, rat, and murine SREBP-1. |
| Background: | Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory elements binding proteins (SREBPs). SREBPs directly activate the expression of over 30 gened involved in teh synthesis and uptake of cholesterol, fatty cids, triglycerides and phopholipids. SREBP-1 acts primarily to activate genes in fatty acid synthesis. Nuclear SREBP-1 upregulates gene expression of a group of a target lipogenic enzymes such as acetyl CoA carboxylase and fatty acid synthase. |
| Detection Method: | Colorimetric |
| Target Species: | Mouse, Rat, Human |
| Tissue Specificity: | Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0422 | SREBP-2 Transcription Factor Assay Kit | Inquiry |
| EKIT-0426 | SREBP-1 Transcription Factor Assay Kit | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0560 | Recombinant Human SRF | Inquiry |
| PE-0561 | Recombinant Human SRF, His-tagged | Inquiry |
| PE-0562 | Recombinant Human SRF, His-tagged | Inquiry |
| Related Gene / Proteins | |||
| SRBD1 | SRC1 | SREBP | SREBP-1 |
| SREBP-2 | SRF | SRP14 | SRP19 |
| SRY | |||
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