| Cat.No.: | EKIT-0470 |
| Product Name: | JHDM1D (KDM7A) Chemiluminescent Assay Kit |
| Size: | 96 reactions |
| Description: | The JHDM1D Chemiluminescent Assay Kit is designed to measure JHDM1D activity for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JHDM1D enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. |
| Applications: | For studying enzyme kinetics and HTS applications |
| Kit Components: | A convenient format, with a 96-well strip plate precoated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified JHDM1D for 96 enzyme reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
| CL-0089 | Human KDM2B Knockout Cell Line 14bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| JHDM1A | JHDM1B | JHDM1D | JHDM2A |
| JHDM3A | KDM1 | KDM1A | KDM1B |
| KDM2 | KDM2A | KDM2B | KDM3A |
| KDM3B | KDM4 | KDM4A | KDM4B |
| KDM4C | KDM4D | KDM4E | KDM5 More > |
| KDM5A | KDM5B | KDM5C | KDM5D |
| KDM6A | KDM6B | KDM6C | KDM7 |
| KDM7A | KDM7B | KDM8 | |
USA
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