Cat.No.: | EKIT-0472 |
Product Name: | JHDM1D (KDM7A) Homogeneous Assay Kit |
Size: | 384 reactions |
Description: | The JHDM1D Homogeneous Assay Kit is designed to measure activity of JHDM1D for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JHDM1D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. |
Applications: | For studying enzyme kinetics and HTS applications. |
Kit Components: | The JHDM1D Homogeneous Assay Kit comes in a convenient AlphaLISA format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JHDM1D for 384 enzyme reactions. |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Cell Lines | ||
CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
CL-0089 | Human KDM2B Knockout Cell Line 14bp deletion | Inquiry |
◆ Antibodies | ||
EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
Related Gene / Proteins | |||
JHDM1A | JHDM1B | JHDM1D | JHDM2A |
JHDM3A | KDM1 | KDM1A | KDM1B |
KDM2 | KDM2A | KDM2B | KDM3A |
KDM3B | KDM4 | KDM4A | KDM4B |
KDM4C | KDM4D | KDM4E | KDM5 More > |
KDM5A | KDM5B | KDM5C | KDM5D |
KDM6A | KDM6B | KDM6C | KDM7 |
KDM7A | KDM7B | KDM8 |
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