| Cat.No.: | EKIT-0560 |
| Product Name: | JARID1A (KDM5A) Chemiluminescent Assay Kit |
| Size: | 96 reactions |
| Description: | The JARID1A Chemiluminescent Assay Kit is designed to measure JARID1A activity for screening and profiling applications. The key to the JARID1A Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JARID1A enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. |
| Background: | JARID1A is a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. |
| Applications: | For studying enzyme kinetics and HTS applications. |
| Kit Components: | Kit comes in a convenient format, with a 96-well strip plate precoated with methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JARID1A for 100 enzyme reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0002 | AT9283 | Inquiry |
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0017 | Human JARID2 Knockout Cell Line 11bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| EAb-0079 | JARID2 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| JADE1 | JAK | JAK1 | JAK2 |
| JAK3 | JAKMIP1 | JARID | JARID1 |
| JARID1A | JARID1B | JARID1C | JARID2 |
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B More > |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | ||
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