Cat.No.: | EKIT-0563 |
Product Name: | JARID1B (KDM5B) TR-FRET Assay Kit |
Size: | 384 reactions |
Description: | The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading. |
Background: | JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3. |
Applications: | For screening small molecular inhibitors for drug discovery and HTS applications. |
Warning: | For research use only, not for diagnostic use. |
Product Types | ||
◆ Bioactive Small Molecules | ||
BSM-0002 | AT9283 | Inquiry |
◆ Cell Lines | ||
CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
CL-0017 | Human JARID2 Knockout Cell Line 11bp deletion | Inquiry |
◆ Antibodies | ||
EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
EAb-0079 | JARID2 Polyclonal Antibody | Inquiry |
Related Gene / Proteins | |||
JADE1 | JAK | JAK1 | JAK2 |
JAK3 | JAKMIP1 | JARID | JARID1 |
JARID1A | JARID1B | JARID1C | JARID2 |
KDM1 | KDM1A | KDM1B | KDM2 |
KDM2A | KDM2B | KDM3A | KDM3B More > |
KDM4 | KDM4A | KDM4B | KDM4C |
KDM4D | KDM4E | KDM5 | KDM5A |
KDM5B | KDM5C | KDM5D | KDM6A |
KDM6B | KDM6C | KDM7 | KDM7A |
KDM7B | KDM8 |
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