Cat.No.: | EL-0106 |
Product Name: | Recombinant Human PCNA Cell Lysate |
Product Overview: | Human PCNA derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer. |
Species: | Human |
Source: | Baculovirus-Insect Cells |
Lysis Buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Recommended Usage: | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage: | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Warning: | For research use only, not for diagnostic or therapeutic use. |
Gene ID NCBI: | 5111 |
Official Symbol: | PCNA |
Official Full Name: | proliferating cell nuclear antigen |
Synonyms: | PCNA; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein; MGC8367; |
mRNA Refseq: | NM_002592 |
Protein Refseq: | NP_002583 |
MIM: | 176740 |
UniProt ID: | P12004 |
Chromosome Location: | 20pter-p12 |
Function: | DNA binding; DNA polymerase processivity factor activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; identical protein binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding; transcription factor binding; |
Product Types | ||
◆ Bioactive Small Molecules | ||
BSM-0039 | Embelin | Inquiry |
BSM-0108 | Curcumin, Curcuma longa (High Purity) | Inquiry |
BSM-0133 | Garcinol | Inquiry |
◆ Antibodies | ||
EAb-0060 | PCAF Polyclonal Antibody | Inquiry |
◆ Extracts & Lysates | ||
EL-0106 | Recombinant Human PCNA Cell Lysate | Inquiry |
Related Gene / Proteins | |||
PCAF | PCBP2 | PCBP3 | PCBP4 |
PCGF2 | PCGF6 | PCL2 | PCNA |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools