Recombinant Human ANKRD1 293 Cell Lysate


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Cat.No.:  EL-0131
Product Name:  Recombinant Human ANKRD1 293 Cell Lysate
Description:  Antigen standard for ankyrin repeat domain 1 (cardiac muscle) (ANKRD1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Species:  Human
Source:  HEK 293 Cells
Components:  This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Storage:  Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C.
Warning:  For research use only, not for diagnostic or therapeutic use.
Gene ID NCBI:  27063
Official Symbol:  ANKRD1
Official Full Name:  ankyrin repeat domain 1 (cardiac muscle)
Synonyms:  ANKRD1; ankyrin repeat domain 1 (cardiac muscle); ankyrin repeat domain-containing protein 1; ALRP; C 193; CARP; CVARP; MCARP; liver ankyrin repeat domain 1; cardiac ankyrin repeat protein; cytokine-inducible nuclear protein; cytokine-inducible gene C-193 protein; C-193; bA320F15.2;
mRNA Refseq:  NM_014391
Protein Refseq:  NP_055206
MIM:  609599
UniProt ID:  Q15327
Chromosome Location:  10q23.33
Function:  DNA binding; R-SMAD binding; RNA polymerase II transcription coactivator activity; RNA polymerase II transcription factor binding; histone deacetylase binding; p53 binding; protein binding; titin binding; transcription corepressor activity;

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