| Cat.No.: | EAb-0165 |
| Product Name: | BMI1 Polyclonal Antibody |
| Product Overview: | Polyclonal antibody to detect BMI 1 in human, mouse and rat samples. |
| Antibody Type: | Polyclonal |
| Immunogen: | Recombinant protein of human BMI1 |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | BMI1 |
| Purification: | Affinity purified |
| Appearance: | Colorless liquid |
| Formulation: | 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3 |
| Applications: | Western blot, Immunohistochemistry |
| Recommended Dilutions/Conditions: |
Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | Western Blot: K562 cell lysates |
| Species Reactivity: | Human, mouse, rat |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Accession: | P35226 |
| Alternative Name: | RNF51, PCGF4 |
| Scientific Background: | The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long term gene silencing through epigenetic chromatin modifications. The first complex, EEDEZH2, is recruited to genes by DNAbinding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex. Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119. Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0033 | Human COMMD3-BMI1 Knockout Cell Line | Inquiry |
| CL-0034 | Human BMI1 Knockout Cell Line 11bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0164 | BMI1 Polyclonal Antibody | Inquiry |
| EAb-0165 | BMI1 Polyclonal Antibody | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0319 | PTC-209 | Inquiry |
| Related Gene / Proteins | |||
| BMI1 | |||
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