| Cat.No.: | PE-1433 |
| Product Name: | Recombinant Mouse Kdm5b, GST-tagged |
| Product Overview: | Recombinant mouse KDM5B (1-753) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Description: | KDM5B is a lysine (K)-specific demethylase 5B which is highly expressed in testis and shows the lowest expression in skeletal muscle. KDM5B is a histone H3 lysine 4 (H3K4) demethylase up-regulated in prostate cancer and associated with androgen receptor where it regulated its transcriptional activity in reporter gene assays. Overexpression of KDM5B in HeLa cells results in loss of tri-, di-, and monomethyl H3K4, but does not alter the methylation status of H3K9 or H3K36. A KDM5B containing highly conserved putative DNA/chromatin binding motif is specifically up-regulated in breast cancer. |
| Applications: | Western Blot |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | ~112 kDa |
| Purity: | >90% by densitometry |
| Species: | Mouse |
| Formulation: | Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Tag: | GST |
| Expression System: | Sf9 Insect Cells |
| Concentrations: | 0.1 μg/μl |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | Kdm5b lysine (K)-specific demethylase 5B [ Mus musculus ] |
| Gene ID NCBI: | 75605 |
| Synonyms: | KDM5B; lysine (K)-specific demethylase 5B; lysine-specific demethylase 5B; histone demethylase JARID1B; putative DNA/chromatin binding motif 1; jumonji/ARID domain-containing protein 1B; jumonji, AT rich interactive domain 1B (Rbp2 like); PUT1; Plu1; PLU-1; Rb-Bp2; Jarid1b; AW556288; RBBP2H1A; mKIAA4034; D1Ertd202e; 2010009J12Rik; 2210016I17Rik; KIAA4034; |
| mRNA Refseq: | NM_152895 |
| Protein Refseq: | NP_690855 |
| Function: | DNA binding; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-dimethyl-K4 specific); histone demethylase activity (H3-trimethyl-K4 specific); metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; transcription corepressor activity; zinc ion binding; |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
| CL-0089 | Human KDM2B Knockout Cell Line 14bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A More > |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | ||
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