Creative BioMart perfectly combines the methylation DNA immunoprecipitation (MeDIP) technology with real-time quantitative PCR technology to interrogate the exact value of the methylation level of specific regions over a wide range. First, methylated DNA fragments are enriched by specific antibodies, and then detected by real-time quantitative PCR. And the finally quantitative analysis is carried out using Ct value. Since real-time PCR can be performed in 96-well or 384-well format with minimal reaction volume and primers can be synthesized at minimal cost, MeDIP-qPCR is an attractive strategy.

Features of MeDIP-qPCR

1. Utilizing the high sensitivity and specificity of antibodies
2. Real-time quantitative PCR primers are designed for gene promoters or CpG islands of interest
3. Extremely wide linear range spanning seven orders of magnitude
4. High accuracy, repeatability, and sensitivity
5. Cost-effective technique

Our Advantages

1. Tolerance of multiple sample types: we currently accept a wide range of biological and clinical samples including cells and tissues. Sample sources include human, animals, plants, and microorganisms.
2. One-stop service: customers only need to provide samples, and we will complete all the service process from MeDIP, real-time quantitative PCR detection to data analysis. We will provide a complete report, including raw and analytical data, graphs, detailed protocols, and a summary of findings.
3. Stringent quality control: Creative BioMart adds quality control at each critical step in MeDIP-qPCR. Fully monitor MeDIP-qPCR to ensure customers get reliable data.
4. Professional data processing: Creative BioMart has a professional MeDIP-qPCR data processing flow. a) The total input DNA sample is utilized as the template, and the specific primers of the gene to be tested are utilized for qPCR detection to obtain Ct (input); b) MeDIP DNA sample is detected as a template to obtain Ct (IP); c) Calculate the efficiency of MeDIP of the gene detected by the formula %(MeDIP / Total input) = 2^[ Ct (input) – Ct (IP) ] × 100.

Workflow of MeDIP-qPCR at Creative BioMart

Figure 1. Workflow of MeDIP-qPCR at Creative BioMart

If you have any special requirements or questions in the MeDIP-qPCR service, please feel free to contact us. We are looking forward to working together with your attractive projects.