| Cat.No.: | PE-1998 |
| Product Name: | DOT1L Protein, Human, Recombinant |
| Product Overview: | Human DOT1L (NP_115871.1) N-terminal segment (Gly 2-Lys 416) with two additional amino acids (Gly & Pro) at the N-terminus. |
| Background: | Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to theDOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner. |
| Applications: | ELISA; Western Blot , Enzyme activity assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | The recombinant human DOT1L comprises 417 amino acids and has a predicted molecular mass of 47.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions as predicted. |
| Purity: | > 90 % as determined by SDS-PAGE |
| Species: | Human |
| Formulation: | Lyophilized from sterile 20mM HEPES, 150mM NaCl, 1mM EDTA, 15% glycerol, pH 7.51. |
| Accession#: | NP_115871.1 |
| Alternative Names: | DOT1 Protein, Human; KMT4 Protein, Human |
| Expression System: | E. coli |
| Common Name: | DOT1-like histone H3K79 methyltransferase |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
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