| Cat.No.: | EL-0139 |
| Product Name: | Recombinant Human DOT1L 293 Cell Lysate |
| Description: | Antigen standard for DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
| Species: | Human |
| Source: | HEK 293 Cells |
| Components: | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
| Storage: | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Gene ID NCBI: | 84444 |
| Official Symbol: | DOT1L |
| Official Full Name: | DOT1-like, histone H3 methyltransferase |
| Synonyms: | DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone H3-K79 methyltransferase; DKFZp586P1823; |
| mRNA Refseq: | NM_032482 |
| Protein Refseq: | NP_115871 |
| MIM: | 607375 |
| UniProt ID: | Q8TEK3 |
| Chromosome Location: | 19p13.3 |
| Function: | DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transcription factor binding; transferase activity; |
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