| Cat.No.: | PE-3469 |
| Product Name: | Recombinant IKKβ protein |
| Product Overview: | Recombinant human IKKβ protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001547.1) with an N-terminal FLAG tag. The molecular weight of the protein is 87.8 kDa. |
| Background: | IKKβ (Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta), also known as IKBKB, I-Kappa-B-Kinase 2, IKK-Beta and NFKBIKB, is a serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, IKKβ can also phosphorylate several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. IKKβ can phosphorylate FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. |
| Applications: | Suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. |
| Tag: | FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0052 | Human IKBKG Knockout Cell Line 269bp insertion | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0323 | BMS 345541 (trifluoroacetate salt) | Inquiry |
| ◆ Antibodies | ||
| EAb-1951 | IKKγ Monoclonal Antibody | Inquiry |
| EAb-1952 | IKKi/IKKε Polyclonal Antibody | Inquiry |
| EAb-1953 | IKKβ Monoclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| Ikaros | IKBKG | IKK | |
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