| Cat.No.: | EL-0346 |
| Product Name: | THP-1 nuclear extract (TPA stimulated) |
| Description: | THP-1 (TPA stimulated) nuclear extract was prepared from a cell culture of the human acute monocytic leukemia THP-1 cell line. The THP-1 cell line was originally derived from the peripheral blood of a 1 year old body with acute monocytic leukemia. THP-1 cells share similar properties to human monocyte-derived macrophages. These cells differentiate into mature macrophages (phagocytes) when grown in the presence of DMSO or phorbol 12-myristate 13-acetate (TPA, also known as PMA). When stimulated with DMSO or vitamin D3, THP-1 cells adhere to the walls of cell culture flasks, and are therefore frequently utilized as in vitro model systems to study foam cell formation in relation to atherosclerosis. The THP-1 cell line is characterized by the expression of Fc and C3b receptors, the production of IL-1, and the lack expression of cytoplasmic and surface immunoglobulins. In addition, these cells are phagocytic, produce lysosymes and stain positive for alpha-napthyl butyrate esterase. Because of these properties, they are often used in research to study monocyte differentiation. |
| Species: | Human |
| Source: | Human monocytes |
| Components: | 2 x 100 µg of THP-1 nuclear extract (PMA stimulated) at 2.5 µg/µl. |
| Extract Composition: | THP-1 nuclear extract was collected in Lysis Buffer after a 24-hour incubation with PMA (10 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. |
| Application Notes: | THP-1 nuclear extract (TPA stimulated) is recommended for use in studies related to 1) blood monocytes, 2) monocyte differentiation and 3) atherosclerosis. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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