Cat.No.: | EL-0351 |
Product Name: | Saos-2 nuclear extract |
Description: | Saos-2 nuclear extract was prepared from cell cultures of the human sarcoma osteogenic Saos-2 cell line. This non-transformed cell line was derived from the primary osteosarcoma of an 11-year old Caucasian female patient. Saos-2 cells exhibit many characteristic osteoblastic properties, including high levels of alkaline phosphatase expression, parathyroid hormone-mediated induction of adenylate cyclase production, and production and secretion of type I collagen and mineralized matrix. Therefore, they are commonly used to study osteoblastic phenotype-related cellular functions and products and in research related to late-stage osteoblastic differentiation. |
Species: | Human |
Source: | Human osteosarcoma cells |
Components: | 2 x 100 µg of Saos-2 nuclear extract at 2.5 µg/µl. |
Extract Composition: | Saos-2 nuclear extract is supplied in 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. |
Application Notes: | Saos-2 nuclear extract is specifically recommended for studies related to osteoblasts and osteoblastic differentiation. |
Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
Warning: | For research use only, not for diagnostic or therapeutic use. |
Product Types | ||
◆ Extracts & Lysates | ||
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USA
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