| Cat.No.: | EL-0356 |
| Product Name: | PC-12 nuclear extract (Forskolin stimulated) |
| Description: | PC-12 nuclear extract (Forskolin stimulated) was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. This cell line originated from a catecholamine-secreting tumor (pheochromocytoma) of the rat adrenal medulla. In addition to catecholamine, PC-12 cells also produce neurotransmitters. PC-12 cells are frequently used as a model system for neuronal differentiation because of their ability to differentiate into a sympathetic neuronal phenotype in response to nerve growth factor, fibroblast growth factor and, under certain conditions, epidermal growth factor. Forskolin is a diterpene compound that activates adenylyl cyclase, thus elevating intracellular levels of cAMP. Activation of the cAMP signaling pathway is associated with the regulation of a number of cellular processes, including motility, neurite outgrowth, metabolism, transcription, and neurotransmitter secretion. Similar to nerve growth factor stimulation of the MAPK signaling pathway, forskolin stimulation leads to PKA-dependent phosphorylation and activation of the CREB transcription factor. In the brain, CREB function is primarily associated with long-term potentiation in the formation of memories. Disturbance of CREB function in the brain is linked to the progression of Huntington's Disease and Rubinstein-Taybi syndrome, as well as some forms of cancer. |
| Species: | Rat |
| Source: | Rat adrenal gland; pheochromocytoma (kidney tumor) |
| Components: | 2 x 100 µg of PC-12 nuclear extract (Forskolin stimulated) at 2.5 µg/µl. |
| Extract Composition: | PC12 nuclear extract was collected in Lysis Buffer after a 30-minute incubation with forskolin (20 µM). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay. |
| Application Notes: | PC-12 nuclear extract (Forskolin stimulated) is specifically recommended for studies related to 1) neuronal development, 2) neurobiology, 3) neurobiochemistry, and 4) neurotoxicity. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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USA
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