NIH/3T3 nuclear extract


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Cat.No.:  EL-0363
Product Name:  NIH/3T3 nuclear extract
Description:  NIH/3T3 nuclear extract was prepared from a cell culture of the NIH/3T3 mouse fibroblast cell line obtained from Swiss mouse embryo tissue. This cell line was established from immortalized primary mouse embryonic fibroblast cells that were cultured by the '3T3 protocol'. 3T3 (3-day transfer, inoculum 3 x 105 cells) refers to the transfer and inoculation protocol used to initiate and propagate the cell line. In research, these cells have become the standard mouse fibroblast cell line.
Species:  Mouse
Source:  Mouse embryo
Components:  200 µg of NIH/3T3 nuclear extract at 2.5 µg/µl.
Extract Composition:  NIH/3T3 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
Application Notes:  NIH/3T3 nuclear extract is recommended for use across a wide range of applications and disciplines requiring analysis of a standard mouse fibroblast cell line.
Storage:  Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Warning:  For research use only, not for diagnostic or therapeutic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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