| Cat.No.: | EL-0369 |
| Product Name: | MCF-7 nuclear extract (H2O2 post-treated) |
| Description: | MCF-7 nuclear extract (H2O2 post-treated) was prepared from cell cultures of the human MCF-7 breast adenocarcinoma cancer cell line. The MCF-7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer. MCF-7 cells are the best characterized and most commonly utilized cell lines in in vitro breast cancer studies. The MCF-7 cell line has retained several characteristics that are particular to mammary epithelium, including the ability to grow as a monolayer, form domes, and respond to hormones. These cells also display a low metastatic potential, leading to the assumption that they represent an early epithelial adenocarcinoma phenotype. These characteristics make MCF-7 cells an ideal model system to study malignant progression in relation to breast cancer. Treatment of MCF-7 cells with reactive oxygen species (ROS), such as H2O2 triggers an oxidative stress response that leads to toxicity and eventual apoptosis. H2O2 signals an antiproliferative/repair response that induces antioxidant systems to metabolize H2O2 to avert ROS toxicity. |
| Species: | Human |
| Source: | Human breast carcinoma |
| Components: | 2 x 100 µg of MCF-7 nuclear extract (H2O2 post treated) at 2.5 µg/µl. |
| Extract Composition: | MCF-7 nuclear extract was collected in Lysis Buffer. Post-collection treatment consists of exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 minutes on ice. The Lysis Buffer consists of 20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT. The protein content has been determined by a Bradford-based assay. |
| Application Notes: | MCF-7 nuclear extract (H2O2 post-treated) is recommended for studies related to 1) breast cancer research, 2) cellular response to oxidative stress and 3) apoptosis. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
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