| Cat.No.: | EL-0378 |
| Product Name: | Jurkat nuclear extract (Heat Shock) |
| Description: | Jurkat nuclear extract (Heat Shock) was prepared from cell cultures of the Jurkat human CD4(+) T cell lymphoblast-like cell line. The Jurkat cell line was established from the propagation of peripheral blood cells of a 14 year old boy with T cell leukemia. These cells are primarily used in research as a T cell model system, particularly in studies of T cell signaling, T cell leukemia, and response to viral infection. Jurkat cells are frequently used in HIV-related research to examine the mechanisms governing T cell-mediated immunity in response to HIV infection. Stimulation of these cells with phorbol esters and either lectins or monoclonal antibodies leads to activation of T cell antigen receptors and induction of IL-2 expression. Because these cells can produce IL-2 in response to stimulation, they are frequently used in research to study the regulation of cytokine expression and differentiation. However, the primary use of Jurkat cells is to study the response of various cancers to radiation or drug treatments. Heat shock treatment of Jurkat cells induces a stress response that subsequently leads to apoptosis. Heat shock treated Jurkat cell lines are frequently used to study the physiological response of cells to heat stress and the processes leading to stress-induced apoptosis. |
| Species: | Human |
| Source: | Human T lymphoblast; acute T cell leukemia |
| Components: | 200 µg of Jurkat nuclear extract (Heat Shock treated) at 3.97 µg/µl. |
| Extract Composition: | Jurkat nuclear extract (Heat Shock) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). The nuclear extract was prepared from the heat shock-treated Jurkat human T cell leukemia cell line (clone E6-1). Cells were cultured in medium that was heated at 45°C for 45 minutes immediately prior to harvesting. |
| Application Notes: | Jurkat nuclear extract (Heat Shock) is specifically recommended for use in studies related to 1) T cell-mediated immunity and response to viral infection (e.g. HIV), 2) cytokine induction and cell differentiation, 3) cellular response to cancer therapies, and 4) heat stress and stress-induced apoptosis. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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