| Cat.No.: | EL-0380 |
| Product Name: | K-562 nuclear extract (TPA stimulated) |
| Description: | K-562 nuclear extract (TPA stimulated) was prepared from a cell culture of the K-562 human myelogenous leukaemia cell line. The K-562 cell line was originally derived from immortalized erythroleukaemia cells obtained from a patient with a Philadelphia chromosome-positive chronic myeloid leukemia in blast crisis. These pluripotent cells are widely applicable in research due to their ability to spontaneously differentiate into erythrocyte, granulocyte and monocyte progenitors. K-562 cells are also frequently utilized in in vitro natural killer (NK) cell assays as highly sensitive targets since they lack the expression of MHC class I molecules required for protection against NK activity. Treatment of K-562 cells with the potent tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to various biological changes that mimic those observed in cells transformed by chemical carcinogens or viruses. Tumor promoters such as TPA are thought to play a significant role in the critical first step in multistage tumorigenesis known as tumor conversion. Treatment of K-562 cells with TPA leads to induction of megakaryocytic differentiation and decreased susceptibility to NK activity. Thus, TPA stimulated K-562 cells are frequently utilized in cancer research as a model system for studying the radiomimetic and tumor-promoting effects of TPA on the differentiation stage of human malignant cells. |
| Species: | Human |
| Source: | Human lymphoblast bone marrow; chronic myelogenous leukemia (CML) |
| Components: | 2 x 100 µg of K-562 nuclear extract (TPA stimulated) at 2.5 µg/µl. |
| Extract Composition: | K-562 nuclear extract (TPA stimulated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12-myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting. |
| Application Notes: | K-562 nuclear extract is recommended for use in studies related to 1) differentiation, 2) immunology, and 3) cancer. K-562 nuclear extract (TPA stimulated) is specifically recommended for analysis of the mechanisms underlying tumorigenesis or TPA-mediated tumor conversion and its associated biochemical responses, including increased membrane metabolism, induction of protein biosynthesis, and alterations in the cell cycle. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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