| Cat.No.: | EL-0389 |
| Product Name: | HT-29 nuclear extract |
| Description: | HT-29 nuclear extract was prepared from a cell culture of the human colorectal adenocarcinoma HT-29 cell line. The HT-29 cell line was originally derived from intestinal tumor tissue obtained from a 44 year old Caucasian female patient with colorectal adenocarcinoma. HT-29 cells are morphologically epithelial, are CD4 negative, and produce carcinoembryonic antigen (CEA) and immunoglobulin A (IgA). This cell line contains a mutation in codon 273 of the p53 gene that results in an Arg>His substitution. In the absence of carbohydrates, HT-29 cells differentiate into polarized epithelium and, therefore, have been used as in vitro model systems to study epithelial differentiation. However, the HT-29 cell line is most commonly used in research as a model for colorectal cancer in studies related to growth and tumorigenicity. |
| Species: | Human |
| Source: | Human epithelial colon; colorectal adenocarcinoma |
| Components: | 200 µg of HT-29 nuclear extract at 5.6 µg/µl. |
| Extract Composition: | HT-29 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). |
| Application Notes: | HT-29 nuclear extract is recommended for use in studies related to colorectal cancer and epithelial differentiation. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
