| Cat.No.: | EL-0395 |
| Product Name: | HeLa nuclear extract (TPA stimulated, 10 min) |
| Description: | HeLa nuclear extract (TPA stimulated, 10 min) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line. Treatment of HeLa cells with the potent tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to various biological changes that mimic those observed in cells transformed by chemical carcinogens or viruses. In mice, TPA has been shown to induce long lasting effects, including inflammation and hyperplasia, that are associated with the critical first step in multistage tumorigenesis known as tumor conversion. Thus, TPA stimulated HeLa cells are frequently utilized in cancer research as a model system for studying the radiomimetic and tumor-promoting activity of TPA on replicating cells. |
| Species: | Human |
| Source: | Human Cervix Adenocarcinoma |
| Components: | 2 x 100 µg of HeLa nuclear extract (TPA stimulated, 10 min) at 2.5 µg/µl. |
| Extract Composition: | HeLa nuclear extract (TPA stimulated, 10 min) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 62 ng/ml TPA (phorbol, 12-myristate, 13 acetate) for 10 minutes at 37°C immediately prior to harvesting. |
| Application Notes: | HeLa nuclear extract (TPA stimulated, 10 min) is specifically recommended for analysis of the mechanisms underlying tumorigenesis or TPA-mediated tumor conversion and its associated biochemical responses, including increased membrane metabolism, induction of protein biosynthesis, and alterations in the cell cycle. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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USA
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