| Cat.No.: | EL-0400 |
| Product Name: | HeLa nuclear extract (2 hr serum response) |
| Description: | HeLa nuclear extract (2 hr serum response) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line. The addition of serum to HeLa cells after serum starvation stimulates various biological processes associated with induction of gene expression, differentiation, proliferation, and apoptosis. Therefore, serum-stimulated cells are commonly utilized in research to analyze activation of transcription of early response genes. It has been shown that serum stimulation leads to activation of several key transcription factors, including activator protein 1 (AP-1) family and Sp1 that regulate the expression of early response genes. |
| Species: | Human |
| Source: | Human Cervix Adenocarcinoma |
| Components: | 200 µg of HeLa nuclear extract (2 hr serum response) at 4.3 µg/µl. |
| Extract Composition: | HeLa nuclear extract (2 hr serum response) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured for 24 hours in low serum (0.5%) conditions and then serum-stimulated (10%) for 2 hours immediately prior to harvesting. |
| Application Notes: | HeLa nuclear extract (2 hr serum response) is specifically recommended for analysis of 1) AP-1 and Sp1 mediated gene expression, 2) differentiation, 3) proliferation, and 4) cell survival. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
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